The lipids of fish. 6. The lipids of cod flesh

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Abstract
Cod flesh was extracted successively with a series of solvents and the various extracts were purified, fractionated and analyzed by the procedures used previously for haddock flesh. Cod flesh contains the same amount of total lipids as haddock flesh (about 0.6%) and the lipid mixture is very similar in the 2 species, that from cod containing approximately lecithin 35, waxes and alcohols 13, free cholesterol 8, phosphatidyl ethanolamine 7, free fatty acids 6, cholesterol esters 5, triglycerides 3, inositol lipids 2 and unidentified lipids 21%. The unidentified lipids of cod flesh resemble those from haddock in containing at least 2 types of phospholipid. One type is apparently based on phosphorylated glycerol but not on normal glycerophosphoric acid, and probably has a fatty acid: glycerol: P ratio approximately 4:2:1. The other type also has a fatty acid: P ratio of about 4:1, but its P: glycerol relationship has not yet been studied. These phospholipids probably contain N, but the bases in question have not been identified. The inositol lipids of both species include more than one type of compound and in the cod such compounds are present in considerably different proportions from those found in haddock-flesh extracts. Hydrocarbons found in both cod- and haddock-lipid extracts are probably contaminants derived from rubber. Complex acidic lipids occur in the cod extracts, as in those from haddock.