Detection of Glycosylated and Deglycosylated Extensin Precursors by Indirect Competitive ELISA

Abstract
A competitive indirect enzyme-linked immunosorbent assay (ELISA) was developed for the rapid quantitation of the glycosylated and deglycosylated forms of the monomeric soluble extensin precursor subunits P1 and P2 [from tomato cell suspension cultures]. A log-linear response range for each kind of precursor in the competition curve was between 0.01 and 100 nanograms per milliliter.