Translating Biomolecular Recognition into Nanomechanics
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- 14 April 2000
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 288 (5464) , 316-318
- https://doi.org/10.1126/science.288.5464.316
Abstract
We report the specific transduction, via surface stress changes, of DNA hybridization and receptor-ligand binding into a direct nanomechanical response of microfabricated cantilevers. Cantilevers in an array were functionalized with a selection of biomolecules. The differential deflection of the cantilevers was found to provide a true molecular recognition signal despite large nonspecific responses of individual cantilevers. Hybridization of complementary oligonucleotides shows that a single base mismatch between two 12-mer oligonucleotides is clearly detectable. Similar experiments on protein A–immunoglobulin interactions demonstrate the wide-ranging applicability of nanomechanical transduction to detect biomolecular recognition.Keywords
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