Surface Recognition of a Protein Using Designed Transition Metal Complexes
- 5 June 2001
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 123 (26) , 6283-6290
- https://doi.org/10.1021/ja003193z
Abstract
Each protein has a unique pattern of histidine residues on the surface. This paper describes the design, synthesis, and binding studies of transition metal complexes to target the surface histidine pattern of carbonic anhydrase (bovine erythrocyte). When the pattern of cupric ions on a complex matches the surface pattern of histidines of the protein, strong and selective binding can be achieved in aqueous buffer (pH = 7.0). The described method of protein recognition is applicable to proteins of known structures. With rapidly increasing number of solved protein structures, the method has wide applicability in purification, targeting, and sensing of proteins.Keywords
This publication has 30 references indexed in Scilit:
- Regioselective Hydrolysis of Tryptophan-Containing Peptides Promoted by Palladium(II) ComplexesJournal of the American Chemical Society, 1999
- The Crystal Structure of an Azide Complex of the Diferrous R2 Subunit of Ribonucleotide Reductase Displays a Novel Carboxylate Shift with Important Mechanistic Implications for Diiron-Catalyzed Oxygen ActivationJournal of the American Chemical Society, 1999
- Interactions of Cytochrome c and Cytochrome f with Aspartic Acid PeptidesJournal of the American Chemical Society, 1999
- Recognition and Stabilization of an α-Helical Peptide by a Synthetic ReceptorJournal of the American Chemical Society, 1997
- Isothermal Titration Microcalorimetric Studies for the Binding of Octenoyl-CoA to Medium Chain Acyl-CoA DehydrogenaseBiochemistry, 1997
- Specific cleavage of histidine‐containing peptides by copper (II)International Journal of Peptide and Protein Research, 1996
- 1H and 113Cd NMR Investigations of Cd2+ and Zn2+ Binding Sites on Serum Albumin: Competition with Ca2+, Ni2+, Cu2+, and Zn2+Inorganic Chemistry, 1996
- Application of Immobilized Metal Ion Chelate Complexes as Pseudocation Exchange Adsorbents for Protein SeparationBiochemistry, 1996
- Enzyme Immobilization on Polymerizable Phospholipid AssembliesPublished by American Chemical Society (ACS) ,1994
- Molecular weight dependence of the critical strain rate for flexible polymer solutions in elongational flowMacromolecules, 1992