DNA From Plant Mitochondria

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Abstract
DNA was isolated from a mitochondrial fraction of each of the following plant materials: Mung bean (Phaseolus aureus) etiolated hypocotyl; turnip (Brassica rapa root; sweet potato (Ipomoea batatas) root; and onion (Allium cepa) bulb. CsCl density gradient centrifugation analysis of the DNA revealed that all of these mitochondrial fractions contained DNA, whose densities were identical (P =1.706 g.cm-3) but different from that of the respective nuclear DNA. Both Phaseolus and Brassica mitochondrial DNA''s showed the hyperchromicity characteristic of double stranded DNA upon heating; Tm''s in 0.0195 N+ were the same; 72.0[degree]. The Phaseolus mitochondrion was estimated to contain 5 x 10-10 [mu]g DNA; Brassica and Ipomoea were approximately the same.