Sister chromatid exchange in highly purified human B and T lymphocytes
- 1 February 1986
- journal article
- conference paper
- Published by Springer Nature in Human Genetics
- Vol. 72 (2) , 160-163
- https://doi.org/10.1007/bf00283937
Abstract
Sister chromatid exchange (SCE) rates were determined in human peripheral blood B and T lymphocyte populations highly purified by immunologic methods. The purified populations were supplemented with γ-irradiated unseparated autologous mononuclear cells to restore helper-functions and stimulated with pokeweed mitogen (PWM) and phytohemagglutinin (PHA), respectively. Measured at the different peaks of proliferation after identical bromodeoxyuridine (BrdU) incubation times, T lymphocytes showed significantly higher SCE frequencies than B lymphocytes. In both populations, different proliferation kinetics and a different minimal BrdU concentration for sister chromatid differentiation (SCD) were observed.Keywords
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