Abstract
Protoplasts of Bacillus licheniformis, prepared by the action of lysozyme, form bacitracin in amounts comparable to whole cells. Antibiotic formation requires the addition of six of the ten constituent amino acids. The formation of antibiotic is inhibited by the addition of D-phenylalanine and this inhibition is released by added L-phenylalanine. These results indicate that the formation of bacitracin involves the utilization of L-phenylalanine.

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