Enzyme-linked protein A: an enzyme-linked immunosorbent assay reagent for detection of human immunoglobulin G and virus-specific antibody

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Abstract
A general-purpose reagent capable of reacting with IgG in a modified enzyme-linked immunosorbent assay [ELISA] technique was prepared by using protein A coupled with horseradish peroxidase. The reagent detected low levels (0.003-1.0 .mu.g/ml) of human IgG and was also applied in an ELISA for titration of antibody to human cytomegalovirus. The antibody titers to human cytomegalovirus determined by ELISA and by complement fixation [CF] were compared. The correlation between the 2 techniques was r = 0.85, but the ELISA was 10 times more sensitive than CF in terms of antibody titers detected.