EFFECT OF MAGNESIUM-DEPENDENT CELL-MEMBRANE ALTERATIONS ON THE TRANSPORT OF K+ IN EHRLICH ASCITES TUMOR-CELLS
- 1 January 1980
- journal article
- research article
- Vol. 39 (2-3) , 177-184
Abstract
Mg-deficiency or Mg-loading of tumor cells changes the permeability of the cell membrane. The influence of this change on the K+ transport across the membrane was investigated using 86Rb+ as K+ analog. The time course of the influx and efflux rates were estimated by means of a mathematical approach for a 2-compartment system with inconstant pool sizes. The comparison of the 2 states of the cells demonstrates that in Mg-deficient cells the passive K+ efflux is significantly enhanced (40%). This in turn stimulates the active counter transport mediated by the (Na+-K+)ATPase, raising the ATP consumption by about 30%. However, the enzyme is not able to maintain the cellular K+ content under these conditions. After a short, transient increase due to the initially enhanced influx the passive net efflux prevails. Differences in the electrophoretic mobility of the 2 states of the cells confirm Mg-dependent changes of the cell membrane structure.This publication has 3 references indexed in Scilit:
- EFFECT OF MAGNESIUM ON ATP-GENERATING AND ATP-UTILIZING REACTIONS IN EHRLICH ASCITES TUMOR-CELLS1979
- Correlated effects of external magnesium on cation content and DNA synthesis in cultured chicken embryo fibroblastsJournal of Cellular Physiology, 1977
- UNTERSUCHUNGEN UBER DEN ELEKTROLYTHAUSHALT DER EHRLICH-ASCITES-TUMORZELLE1963