Identification of the second major allergen of Japanese cedar pollen
- 1 May 1990
- Vol. 45 (4) , 309-312
- https://doi.org/10.1111/j.1398-9995.1990.tb00501.x
Abstract
We isolated and characterized the second major allergen (Cry j II) from Japanese cedar pollen. We found that most patients with this pollinosis had IgE antibody to this protein in addition to IgE antibody to Cry j I; however, some sera reacted only with Cry j I or Cry j IL IgE-ELISA inhibition studies revealed that Cry j I and Cry j II had no cross-allergenicity. Cry j II did not react with anti-Cry j I monoclonal antibodies. In SDS-PAGE under a non-reducing condition. Cry j II showed a band at the 37 kDa position, compared with the 45–50 kDa bands of Cry j I. N-terminal amino acid sequence of Cry j II was completely different from that oi Cry j I.Keywords
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