Kinetics of RNA synthesis in toad bladder epithelium: action of aldosterone during the latent period
Open Access
- 1 March 1971
- journal article
- Published by American Society for Clinical Investigation in Journal of Clinical Investigation
- Vol. 50 (3) , 543-551
- https://doi.org/10.1172/jci106523
Abstract
Processing of RNA in the toad bladder was analyzed by polyacrylamide-gel electrophoresis to determine whether aldosterone causes any changes in the 1 hr before it potentiates transport of sodium ion. No change was found in the quantity or in the specific activity of bulk RNA labeled with uridine-5-3H. In vivo and in vitro with either uridine-5-3H or with methionine-(methyl)-3H as precursors, processing of RNA was extremely slow. Heterodisperse RNA was obvious after 30 min of continuous labeling, but labeling of the 40S precursor of ribosomal RNA was not apparent for 60 min. Labeling of mature 28S and 18S RNA first became apparent after 8 hr. ∼7S RNA was the principal fastmigrating species labeled at 30 min, and 4S RNA was not heavily labeled until 1 hr. Aldosterone (5 × 10-7 mole/liter) produced no changes. If care were not taken to inhibit metabolism of native bacteria colonizing the bladder, bacterial RNA of high specific activity predominated. We conclude that RNA metabolism in the toad bladder is extraordinarily slow, that a major acceleration of de novo synthesis in response to physiologic doses of aldosterone was not demonstrable, and that some reports to the contrary may have been influenced by artifacts from bacterial RNA metabolism. Earlier evidence for obligatory alterations in RNA metabolism during the latent period is not strong.Keywords
This publication has 47 references indexed in Scilit:
- METABOLISM OF RIBOSOMAL PRECURSOR RIBONUCLEIC ACID IN KIDNEYThe Journal of cell biology, 1970
- Pool size and specific activity of UTP in isoproterenol-stimulated salivary glandsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969
- Labeling of RNA in the perfused heart: The problem of bacterial contaminationBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969
- GIANT NUCLEOPLASMIC RNA IN THE SWITCH-ON OF COMPENSATORY RENAL GROWTHProceedings of the National Academy of Sciences, 1969
- Time required by the normal and regenerating rat liver cell to make a ribosomeJournal of Molecular Biology, 1968
- Mechanism of action of aldosteronePhysiological Reviews, 1966
- DEGRADATION OF RIBONUCLEIC ACID IN MOUSE FIBROBLASTS TREATED WITH ACTINOMYCINThe Journal of cell biology, 1965
- Mechanism of the antinatriuretic action of aldosteroneBiochemical Pharmacology, 1963
- ON THE MECHANISM OF ACTION OF ALDOSTERONE ON SODIUM TRANSPORT: THE ROLE OF PROTEIN SYNTHESISProceedings of the National Academy of Sciences, 1963
- Chemical Nature of the System controlling Gene Activities in Insect CellsNature, 1963