Influence of sulfide compounds on the metabolism of Methanobacterium strain AZ

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Abstract
Various organic sulfides and inorganic sulfide were studied in respect to their effect on growth and methane production of Methanobacterium strain AZ. In mineral, sulfide-free medium, cysteine regulated the specific rate of methane production (optimum concentration =5·10−4 mole/l). A supplement of sulfide (10−4 mole/l) caused an additional stimulation. Coenzyme M** or glutathione could be substituted for cysteine when sulfide was present. Growth was stimulated by CoM and glutathione to the same extent as with cysteine in sulfide-containing media. The concentration of sulfide in cysteine-containing media affected the excretion of amino acids.