REGULATION OF CELL-MEDIATED CYTOTOXICITY .3. SYNERGISM OF CORTICO-RESISTANT WITH ANTI-THYMOCYTE SERUM-RESISTANT SPLENIC T CELLS IN GENERATION OF CYTOTOXIC LYMPHOCYTES IN GRAFT-VS-HOST REACTIONS

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 119  (2) , 781-785
Abstract
Lethally irradiated F1 hybrid mice were given an i.v. injection of parental strain spleen cells. Six days later, their spleen cells were used as the effector cells to measure the in vitro cell-mediated cytotoxicity (CMC) of the parental cells. The treatment of the donors with hydrocortisone resulted in a marked decrease of the capacity of their spleen cells to produce a CMC reaction, whereas the treatment with anti-thymocyte serum (ATS) resulted in an almost complete loss of such activity. The mixing of spleen cells from hydrocortisone-treated parental donors with the spleen cells from ATS-treated parental donors before injection resulted in a synergistic amplification of the cytotoxic response. The anti-Thy-1 serum treatment of either spleen cell population abolished the synergism completely. Cortico-resistant T [thymus-derived] cells apparently act as precursors of cytotoxic lymphocytes. ATS-resistant T cells probably produce an amplification of their reaction.