Size Fluctuations Can Explain Anomalous Mobility in Field-Inversion Electrophoresis of DNA
- 26 December 1988
- journal article
- research article
- Published by American Physical Society (APS) in Physical Review Letters
- Vol. 61 (26) , 2965-2968
- https://doi.org/10.1103/physrevlett.61.2965
Abstract
We extend the reptation model of DNA gel electrophoresis by replacing the tube by a chain of narrow "straits" connecting open "lakes." The DNA chain can accumulate easily in the lakes, thus allowing large fluctuations in the length of the chain. Unlike the standard model, this model, simulated on a computer, shows a sharp minimum in mobility as the period is varied with periodically reversing fields, similar to experiments.Keywords
This publication has 11 references indexed in Scilit:
- Theoretical Studies of DNA During Gel ElectrophoresisScience, 1988
- Molecular mechanism of field-inversion electrophoresisPhysical Review Letters, 1988
- Electron microscopy of beaded agarose gelsBiopolymers, 1988
- Dynamics of pulsed-field electrophoresisPhysical Review Letters, 1987
- Self-trapping and anomalous dispersion of DNA in electrophoresisPhysical Review Letters, 1987
- Transient orientation of linear DNA molecules during pulsed-fleld gel electrophoresisNucleic Acids Research, 1987
- Electrophoretic Separations of Large DNA Molecules by Periodic Inversion of the Electric FieldScience, 1986
- Prediction of chain elongation in the reptation theory of DNA gel electrophoresisBiopolymers, 1985
- Theory of gel electrophoresis of DNABiopolymers, 1985
- Study of agarose gels by electron microscopy of freeze‐fractured surfacesBiopolymers, 1982