A radioimmunoassay for quantitation of secretory component

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Abstract
Secretory component (SC) isolated from human colostrum was radioiodinated with chloramin-T method and it was subjected to a radioimmunoassay for quantitation of SC. A monospecific rabbit anti-sera against human SC which could react with free SC as well as immunoglobulin bound SC was used for the assay. The present method can measure as low as 300ng/ml of SC. The level (mean ±2 S.D.) of serum SC in healthy subjects was determined to be 31.3 ±7.2Μg/ml. Studies with Sephadex G-200 gel filtration demonstrated that SC in the normal sera existed mostly as a form of SC-IgA. In contrast, free SC was never detected in them.