Stability of Vitamins in a Chemically Defined Medium for 929-L Fibroblasts2
- 1 September 1963
- journal article
- research article
- Published by Oxford University Press (OUP) in JNCI Journal of the National Cancer Institute
- Vol. 31 (3) , 603-610
- https://doi.org/10.1093/jnci/31.3.603
Abstract
Thiamine and ascorbic acid, but not riboflavine or choline, were unstable in Waymouth's medium, MB 752/1, stored at 4° or incubated at 36°. Growth of cells in medium at 36°C caused no more loss of vitamin content than did incubation of medium without cells. Ascorbic acid decomposed to products other than dehydroascorbic and diketogulonic acids. Thiamine was converted, at least in part, to a disulfide, which, like homomeric thiamine disulfide, was rapidly reduced by cells to free thiamine.Keywords
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- Environmental influences on the metabolism and composition of cultured cellsJournal of Experimental Zoology, 1959
- Rapid Proliferation of Sublines of NCTC Clone 929 (Strain L) Mouse Cells in a Simple Chemically Defined Medium (MB 752/1)2JNCI Journal of the National Cancer Institute, 1959
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