The phosphorylation of pantothenic acid by Lactobacillus arabinosus 17–5

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Abstract
Dried cells of L. arabinosus catalyse the disappearance of pantothenic acid when incubated with adenosine triphosphate (ATP) and phosphate buffer. Phosphatase treatment results in complete recovery of the pantothenic acid, indicating that the substance is being converted into a phosphoric ester. Sodium fluoride increases the extent of phosphorylation, probably due to its inhibition of ATP-splitting enzymes present in the dried cells. The pantothenic acid derivative that is formed was concentrated and its properties compared with those of synthetic pantothenic acid monophosphates. Hydrolysis by intestinal phosphatase, stability to acid and alkali, and chromatographic properties suggest that the derivative is pantothenic acid 4[image]-phosphate.