Multiplex Real-Time PCR Method To Identify Shiga Toxin Genes stx1 and stx2 and Escherichia coli O157:H7/H − Serotype
Open Access
- 1 October 2003
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 69 (10) , 6327-6333
- https://doi.org/10.1128/aem.69.10.6327-6333.2003
Abstract
A multiplex real-time PCR method to simultaneously detect the stx1 and stx2 genes of Shiga toxin-producing Escherichia coli and a unique conserved single-nucleotide polymorphism in the E. coli O157:H7/H − uidA gene has been developed. There is more than 98.6% sensitivity and 100% specificity for all three gene targets based on a panel of 138 isolates. The PCR efficiencies were ≥1.89, and as few as 6 CFU/reaction could be detected.Keywords
This publication has 45 references indexed in Scilit:
- Rapid Detection of Escherichia coli O157:H7 with Multiplex Real-Time PCR AssaysApplied and Environmental Microbiology, 2002
- Relative expression software tool (REST(C)) for group-wise comparison and statistical analysis of relative expression results in real-time PCRNucleic Acids Research, 2002
- Genetic and Evolutionary Analysis of Mutations in thegusAGene That Cause the Absence of β‐Glucuronidase Activity inEscherichia coliO157:H7The Journal of Infectious Diseases, 2001
- A new mathematical model for relative quantification in real-time RT-PCRNucleic Acids Research, 2001
- Rapid Detection of Enterohemorrhagic Escherichia coli by Real-Time PCR with Fluorescent Hybridization ProbesJournal of Clinical Microbiology, 2001
- Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assaysJournal of Molecular Endocrinology, 2000
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997
- Genetic analysis of uidA expression in enterohaemorrhagic Escherichia coli serotype 0157:H7Microbiology, 1994
- ESCHERICHIA COLI 0157:H7 FROM WATERThe Lancet, 1989
- Hemorrhagic Colitis Associated with a RareEscherichia coliSerotypeNew England Journal of Medicine, 1983