Studies on the Coagulation of Chicken Blood. IV. Adsorption of phi-Factor Activity.

Publisher Website
Google Scholar
Abstract
Detailed studies on the adsorption of p -factor activity from oxalated plasma by a series of adsorbents have revealed that this activity is due to the combined activities of at least five different coagulation factors. Three of the factors were inactive when Russell''s viper venom-cephalin was substituted for thromboplastin in the assay system. The experiments indicated that the minimum amount of adsorbent necessary for complete adsorption of a (group of) [phi] -factor(s) from oxalated plasma, may be regarded as an absolute measure of the concentration(s) of the factor(s). It is suggested that the differentiation of the [phi] -factors obtained by the adsorption technique depends on the presence in the surface of the absorbents of special adsorption sites, differing in number and specificity with the various adsorbents, each causing adsorption of one or a few p-factors only.