Further Improvement of the Enzyme-Linked Antiglobulin Test (ELAT) for Erythrocyte Antibodies

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Abstract
The enzyme-linked antiglobulin test (ELAT) was found to be nine times more sensitive than the direct antiglobulin test (DAT) in detecting erythrocytes sensitized by IgG antibody in vitro. The release of hemoglobin and other interfering substances from the erythrocytes gave falsely high absorbance values which were corrected by the use of a hemolysis blank with each test. ELAT results showed good precision. With the use of the hemolysis blank, the ELAT should prove to be a useful tool for hospital blood blanks in the detection of weakly reactive alloand autoantibodies.