A small RNA virus isolated from Atlantic salmon (Salmo salar)

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Abstract
A previously unknown virus was isolated from juvenile Atlantic salmon (Salmo salar) reared in fresh water. The virus induced syncytia in CHSE-214 cells at 10-20.degree.C. Optimum replication occurred at 15.degree.C. Virions, purified from infected CHSE-214 cells, were hexagonal to round with a mean diameter of 39.5 nm (n = 20 SD = 0.32 nm). Virions did not possess an envelope as shown by resistance to chloroform treatments. Replication was not appreciably inhibited by 50 .mu.g/ml 5-bromo-2''-deoxyuridine indicating that the virus has a RNA genome. Waterborne exposures and intraperitoneal injections of the virus into juvenile Atlantic salmon did not cause mortality nor was virus detectable at 33 and 76 d post-exposure. Similar results were obtained when rainbow trout (Oncorhynchus mykiss) were exposed to waterbone virus.