Speciation of non-covalent nickel species in plant tissue extracts by electrospray Q-TOFMS/MS after their isolation by 2D size exclusion-hydrophilic interaction LC (SEC-HILIC) monitored by ICP-MS
- 8 June 2006
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Journal of Analytical Atomic Spectrometry
- Vol. 21 (7) , 676-683
- https://doi.org/10.1039/b602689c
Abstract
An original approach based on successive size-exclusion and hydrophilic interaction HPLC (HILIC) was developed to purify traces of Ni species from a plant aqueous extract. The degree of purity achieved was for the first time sufficient for the identification, in a natural sample, of a number of non-covalent metal complexes by electrospray Q-TOFMS/MS. Nickel complexes with malate, citrate, histidine, EDTA and nicotianamine (NA) were identified in the roots, xylem, shoots and their protoplasts of a metal hyperaccumulator plant Thlaspi caerulescens. The quantitative recovery of the most stable of these complexes (with EDTA and NA) allowed their quantitative determination by SEC-ICP-MS.Keywords
This publication has 38 references indexed in Scilit:
- Integrated mass spectrometry in (semi-)metal speciation and its potential in phytochemistryTrAC Trends in Analytical Chemistry, 2006
- Constitutively High Expression of the Histidine Biosynthetic Pathway Contributes to Nickel Tolerance in Hyperaccumulator PlantsPlant Cell, 2005
- The use of fast protein liquid chromatography with ICP-OES and ES–MS–MS detection for the determination of various forms of aluminium in the roots of Chinese cabbageAnalytica Chimica Acta, 2005
- Prospects of genetic engineering of plants for phytoremediation of toxic metalsBiotechnology Advances, 2004
- Heavy metal specificity of cellular tolerance in two hyperaccumulating plants, Arabidopsis halleri and Thlaspi caerulescensNew Phytologist, 2004
- Comparative microarray analysis of Arabidopsis thaliana and Arabidopsis halleri roots identifies nicotianamine synthase, a ZIP transporter and other genes as potential metal hyperaccumulation factorsThe Plant Journal, 2003
- Cross‐species microarray transcript profiling reveals high constitutive expression of metal homeostasis genes in shoots of the zinc hyperaccumulator Arabidopsis halleriThe Plant Journal, 2003
- Speciation analysis of nickel in the latex of a hyperaccumulating tree Sebertia acuminata by HPLC and CZE with ICP MS and electrospray MS-MS detectionJournal of Analytical Atomic Spectrometry, 2003
- Accumulation Forms of Zn and Pb in Phaseolus vulgaris in the Presence and Absence of EDTAEnvironmental Science & Technology, 2001
- Free histidine as a metal chelator in plants that accumulate nickelNature, 1996