Synthetic Media for Culture of Certain Hemolytic Streptococci

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Abstract
The essential nature of vitamin B6 for 2 species of hemolytic streptococci was demonstrated. Streptococcus zymogenes will grow luxuriantly in a soln. which contains only known pure chemicals[long dash]glucose, inorganic salts, isoleucine, lysine, tyrosine, cystine (or methionine or inorganic sulfide), arginine, tryptophane, glutamic acid, riboflayin, pantothenic acid, "reduced" iron, and vitamin B6. Strains belonging to the groups B and D of Lancefield could be grown successfully in synthetic medium but members of other Lancefield groups could not. In the absence of glutamic acid or of tryptophane, Strep. zymogenes failed to grow; in the presence of these 2, but in the absence of all other amino acids, no growth was obtained. The simplest effective amino acid mixture was found to be tryptophane, glutamic acid, isoleucine, lysine, arginine, tyrosine and cystine.

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