Control of rat C6 glioma cell proliferation: uncoupling of the inhibitory effects of hydrocortisone hormone in suspension and monolayer cultures.
Open Access
- 1 August 1983
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 97 (2) , 455-458
- https://doi.org/10.1083/jcb.97.2.455
Abstract
A comparative study was made of the effects of the hormone hydrocortisone (Hy) on [rat] C6 glioma cells grown in monolayer and in suspension in cultures. Hy reversibly rendered c6 cells anchorage- and serum-dependent for their growth. In monolayer cultures, Hy inhibited cell cycle traversing exclusively at G1 phase. In agarose suspension, Hy blocked colony development. Hy-resistant variants were selected and isolated in agarose suspension. Examination of these variants showed that cells selected for Hy-resitance in suspension can be Hy sensitive when anchored to a solid substrate. Resistance to Hy in suspension and resistance to it in monolayer culture are apparently 2 independent phenotypes.This publication has 20 references indexed in Scilit:
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