A yeast protein that influences the chromatin structure of UASG and functions as a powerful auxiliary gene activator.
Open Access
- 1 April 1990
- journal article
- research article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 4 (4) , 503-514
- https://doi.org/10.1101/gad.4.4.503
Abstract
GRF2, an abundant yeast protein of Mr approximately 127,000, binds to the GAL upstream activating sequence (UASG) and creates a nucleosome-free region of approximately 230 bp. Purified GRF2 binds to sequences found in many other UASs, in the 35S rRNA enhancer, at centromeres, and at telomeres. Although GRF2 stimulates transcription only slightly on its own, it combines with a neighboring weak activator to give as much as a 170-fold enhancement. This effect of GRF2 is strongly distance-dependent, declining by 85% when 22 bp is interposed between the GRF2 and neighboring activator sites.This publication has 61 references indexed in Scilit:
- Initiation by Yeast RNA Polymerase II at the Adenoviral Major Late Promoter in VitroScience, 1989
- Statistical positioning of nucleosomes by specific protein-binding to an upstream activating sequence in yeastJournal of Molecular Biology, 1988
- A yeast silencer contains sequences that can promote autonomous plasmid replication and transcriptional activationCell, 1987
- Binding of transcription factor TFIID to the major late promoter during in vitro nucleosome assembly potentiates subsequent initiation by RNA polymerase IICell, 1987
- A region flanking the GAL7 gene and a binding site for GAL4 protein as upstream activating sequences in yeastJournal of Molecular Biology, 1985
- Unusual DNA sequences associated with the ends of yeast chromosomesNature, 1984
- Assembly of transcriptionally active 5S RNA gene chromatin in vitroCell, 1982
- Transcription by Eukaryotic RNA Polymerases A and B of Chromatin Assembled in vitroEuropean Journal of Biochemistry, 1979
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970