Transcription by Eukaryotic RNA Polymerases A and B of Chromatin Assembled in vitro
- 1 August 1979
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 98 (2) , 317-327
- https://doi.org/10.1111/j.1432-1033.1979.tb13191.x
Abstract
Chromatin was assembled in vitro from SV-40 DNA form I and the calf-thymus 4 histones H2A, H2B, H3 and H4. Transcription with calf thymus RNA polymerases A and B (I and II) was greatly inhibited. Nucleosomes inhibited both RNA chain initiation and elongation. The inhibition of elongation could be relieved by increasing ionic strength, suggesting that electrostatic interactions between histone octamer and DNA must be broken for RNA polymerase to transcribe DNA organized into nucleosomes.This publication has 55 references indexed in Scilit:
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