Genetic Diversity and Differentiation in Northern Populations of the Tree-Hole Mosquito Aedes hendersoni (Diptera: Culicidae)1

Abstract
Natural populations of Aedes hendersoni Cockerell in Indiana and Michigan were analyzed for electrophoretic variation at 23 structural loci. These populations were polymorphic at 45% of their loci and exhibited a mean heterozygosity of 13.4%. Nei's intraspecific genetic distance (D) was 0.008. Interspecific comparisons with a sympatric sibling species, A. triseriatus Say, produced a D value of 0.48. Five enzymes, all non-regulatory, were found to distinguish unambiguously between the two species. A. triseriatus was the more variable species, but A. hendersoni populations were more heterogeneous. No evidence of introgression was detected between the two species.