Quantification of Hepatitis C Virus in Human Liver and Serum Samples by Using LightCycler Reverse Transcriptase PCR
Open Access
- 1 November 2002
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 40 (11) , 4346-4348
- https://doi.org/10.1128/jcm.40.11.4346-4348.2002
Abstract
A highly sensitive, non-probe-based, real-time quantitative reverse transcriptase PCR was developed for viral load measurements in both serum and liver samples from patients with hepatitis C virus (HCV) infection. With synthetic RNA, the linearity of the approach was conserved over a wide range of HCV copy numbers. There was a strong correlation between hepatic and serum viral load measurements ( r = 0.689, P = 0.004, n = 15), indicating that the level of viremia reflected the amount of virus present in the liver.Keywords
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