A microdialysis procedure for extraction and isolation of corticosteroids from peripheral blood plasma

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Abstract
A method of simultaneous dialysis and extraction of relatively polar steroids from blood was adapted for use with 1 ml samples of peripheral blood plasma, a special microdialysis cell being devised for the purpose. Details of construction and use of the cell are given. Corticosteroids, extracted from plasma by this method, were isolated by ascending chromatography on paper, located by UV contact photography, eluted and measured by a fluorophotometric procedure. Recovery of hydrocortisone added to plasma in amounts of 1 [mu]g/ml averaged 89%. Recovery of 5 ug amounts was considerably less satisfactory. Hydrocortisone levels in 10 samples of femoral arterial blood plasma obtained from dogs averaged 033 [mu]g/ml.