Role of Marine Fungi in the Biochemistry of the Oceans. I. Establishment of Quantitative Technique for Cultivation, Growth Measurement and Production of Inocula

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Abstract
An indigenous, cellulolytic, marine deuteromycete, Culcitalna achraspora, was used to illustrate the establishment of a base-line technique for future biochemical studies on the importance of filamentous fungi in the sea. The basic ingredients of growth media were glucose, ammonium nitrate, dipotassium phosphate, tri8(hydroxymethyl)aminomethane and yeast extract in natural or artificial sea water at pH 7.5. Cultures were grown in shake flasks to the mid-to-late exponential phase, harvested, gravimetrically quantitated, and exhausted in toto in deficient media before requantitation and use as standardized inocula for experimental media. The effect of inoculum age on growth in complete and deficient media and inoculum quantity on growth in complete media are noted. Examples of data precision are presented, as well as an application of the Dunnett "t" test which provided preliminary evidence of a thiamine requirement.