NOVEL METHOD FOR ESTIMATING LABELING INDEX IN CLINICAL SPECIMENS WITH USE OF IMMUNOPEROXIDASE-LABELED ANTINUCLEOSIDE ANTIBODIES

  • 1 January 1977
    • journal article
    • research article
    • Vol. 37  (1) , 323-326
Abstract
The labeling index determined by [3H]thymidine autoradiography in [human and mouse] cells from clinical specimens was compared with the percentage of the cells showing nuclear reactivity to immunoperoxidase labeled antinucleoside antibodies. This nuclear immunoreactivity is specific for denatured or single stranded DNA''s and is detectable almost exclusively during DNA synthesis. Results with the 2 methods showed excellent agreement. The new method allowed rapid accurate assessment of the S phase in tumor cells from freshly isolated aspirated specimens as well as frozen sections, suggesting general applicability to estimation of the labeling index without autoradiography.

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