Abstract
We have used double-barelled ion-sensitive microelectrodes to measure the intracellular pH, pHi, th intracellular Na+ activity, aNai, and the membrane potential in identified glial cells of the central nervous system of the leech Hirudo medicinalis to study the effect of CO2-HCO3-. In the presence of CO2-HCO3- an inwardly directed electrogenic Na+-HCO3- co-transport is stimulated across the glial membrane, which greatly determines the pHi and thereby affects the intracellular buffering power of the glial cells.