Electron microscopic mapping of wheat germ RNA polymerase II binding sites on cloned CaMV DNA
Open Access
- 25 August 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 9 (16) , 3927-3939
- https://doi.org/10.1093/nar/9.16.3927
Abstract
The binding sites of wheat germ RNA polymerase II were mapped on the cloned CaMV genome by observation of enzyme-linear DNA complexes by electron microscopy. Twelve sites are observed. Three of them are relatively stable in the presence of heparin and are found at positions 8–9, 21–23, and 41–44 map units on the physical map of the genome. These positions correspond to AT-rich regions of the viral genome which contain potential promoter sites. These results are discussed with reference to current information on the structure and expression of the CaMV genome.Keywords
This publication has 23 references indexed in Scilit:
- Efficient and selective initiation by yeast RNA polymerase B in a dinucleotide-primed reactionNucleic Acids Research, 1981
- Characterisation of heparin-resistant complex formation and RNA synthesis by wheat germ RNA polymerases I, II and III, in vitro on cauliflower mosaic virus DNABiochemical and Biophysical Research Communications, 1981
- Transcription of simian virus 40 DNA by wheat germ RNA polymerase II. Priming of RNA synthesis by the 3'-hydroxyl of DNA at single strand nicks.Journal of Biological Chemistry, 1980
- Localization of the Binding Sites of Prokaryotic and Eukaryotic RNA Polymerases on Simian Virus 40 DNAEuropean Journal of Biochemistry, 1980
- Specific binding and protection of form II SV40 deoxyribonucleic acid by ribonucleic acid polymerase II from wheat germBiochemistry, 1980
- Transcription in vitro of Cauliflower Mosaic Virus DNA by RNA polymerase I, II and III purified from wheat embryosBiochemical and Biophysical Research Communications, 1979
- Comparative study of calf thymus and wheat germ RNA polymerase II: Stability of initiation complexes and elongation ratesBiochemical and Biophysical Research Communications, 1979
- Replication of cauliflower mosaic virus and transcription of its genome in turnip leaf protoplastsVirology, 1978
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- DNA Viruses of Higher PlantsPublished by Elsevier ,1976