Localization of the Binding Sites of Prokaryotic and Eukaryotic RNA Polymerases on Simian Virus 40 DNA

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Abstract
The binding sites of calf thymus RNA polymerase (B) II, wheat germ RNA polymerase B and of the Escherichia coli RNA polymerase were mapped on the SV40 genome by observation of enzyme-linear DNA complexes by EM. There were 3-4 major sites and several minor sites observed for each enzyme; common binding sites for the 3 enzymes are found in positions 0.17, 0.53 and 0.90 of the viral physical map. Initiation complexes with these enzymes can be stabilized with specific ribodinucleotides and a single ribonucleoside triphosphate. Whereas ApA and ATP greatly enhance the binding of the E. coli enzyme at position 0.17, they stabilize the binding of the eukaryotic enzyme at many sites, some of them located near the origin of replication.