HPLC Determination of Enantiomeric Purity of Protected Amino Acid Derivatives Used in Peptide Synthesis
- 1 August 1994
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 17 (13) , 2759-2775
- https://doi.org/10.1080/10826079408013498
Abstract
An improved RP-HPLC method on ODS-Hypersil column with pre-column derivatization with Marfey's reagent were used to monitor racemization in N-, C- and/or side-chain protected amino acid derivatives by separation of a series of new diastereoisomeric Marfey's compounds. Chromatographic samples were obtained by partial deprotection of different starting materials. In a simple two-step procedure (deprotection and derivatization) the compounds of amino acids formed stable diastereomeric derivatives having facile resolutions.Keywords
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