Acid-Soluble Nucleotides in Cow's and Goat's Milk

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Abstract
Acid-soluble nucleo- tides in cow''s and goat''s milk at different stages of lactation were examined by ion-exchange column chromatography. In goat''s milk CMP5[image], DPN, AMP5[image], GMP5[image], UMP5[image], orotic acid, GDP-mannose, UDP-acetylglucosamine, UDP-acetylgalactosamine, UDP-glucose, UDP-galactose, GDP, UDP, UDP-uronic acid, and other unidentified nucleo-tides were found throughout the lactation period. Active P32 incorporation into these nucleotides was observed following the injection of P32. Milk of a goat with lactation induced by the administration of hormones showed the same chromatographic pattern as that of the milk at normal lactation. With the exception of colostral stage, orotic acid constituted a major part of acid-soluble nucleotides of cow milk. Cow milk also contained CMP5[image], uric acid, AMP5[image], and an unidentified AMP derivative,.

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