Genetic Transformation in Methylobacterium organophilum

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Abstract
Several mutants were isolated from the facultative methylotroph, M. organophilum, using N-methyl-N''-nitro-N-nitrosoguanidine or UV light as mutagens. One of these isolates, a glutamate auxotroph lacking isocitrate dehydrogenase was transformed to prototrophy, using wild-type DNA, at a frequency of 0.5%. Competence and DNA uptake occur only in cultures which are near the end of exponential growth, and maximal transformation requires a DNA concentration of 100 .mu.g ml-1.