Inheritance and linkage relationships of some isozymes of black spruce in New Brunswick

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Abstract
Thirteen polymorphic loci from eleven enzyme systems in black spruce (Piceamariana (Mill.) B.S.P.) were studied by gel electrophoresis using embryos and haploid megagametophyte tissue. Two loci (Mdh-1 and Sdh) showed deviations from the expected 1:1 segregation ratio of megagametophytes from heterozygous maternal plants that were significant at the 1 % level and a third (Aat) at the 5% level. Of the 78 possible pairwise comparisons for the linkage analysis, 58 could be tested for jointly independent segregation. Linkage could be detected between three pairs of loci: Aat–Pgi, Gdh–Idh, and Fum–Pgm.