Alkaline Hydrolysis of Phosphoglycerides on Thin Layer Plates In Situ

Publisher Website
Google Scholar
Abstract
An alkaline hydrolysis method for selective cleavage of the acyl groups in phospholipids is described. The alkaline hydrolysis treatment was achieved with 0.8 N methanolic NaOH by incubation at 37°C in a chamber presaturated with aqueous methanol to saturate the ambient vapor phase and thus maintain the concentration of NaOH. Cleavage of acyl groups was quantitative while cleavage of ether groups was below the level of detection. There was no effect on the alkenyl ether linkages of plasmalogens.