Determination of a Trazadone Metabolite, 1-m-Chlorophenyl- Piperazine in Plasma by Liquid Chromatography

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Abstract
A liquid chromatographic assay was developed for monitoring a trazodone metabolite, 1-m-chlorophenylpiperazine (mCPP) in plasma. Alkalinzed plasma was extracted with methylene chloride/isoamyl alcohol (98:2), and back-extracted with diluted phosphoric acid. The extracts were analyzed by a high carbon load, 20%, C-18, 5 um column with a ternary mobile phase at 45[ddot]C and detection at 254 nm. Retention volumes of mCPP, the internal standard and trazodone were 22.7, 38.0 and 58.7 ml, respectively. A peak, X, eluded closely with mCPP. Peak height ratios of mCPP/internal standard were linearly correlated to mCPP plasma concentrations between 10 to 100 ug/L. Detection limit was 5 ng and recovery was about 50%. Precision studies showed the within-run and day-to-day coefficients of variation were 4.0 and 5.7%, respectively. Patient samples (n = 6) showed substantial amounts of trazodone but only trace amounts of mCPP.