Solid-phase synthesis of a nucleopeptide from the linking site of adenovirus-2 nucleoprotein, -Ser(p5′CATCAT)-Gly-Asp-. Convergent versus stepwise strategy
Open Access
- 1 January 1995
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 23 (20) , 4151-4161
- https://doi.org/10.1093/nar/23.20.4151
Abstract
The synthesis of a nucleopeptide with the sequence-Ser(p5′ CATCAT)-Gly-Asp- has been undertaken by either convergent or stepwise solid-phase strategies, both of which use base-labile permanent protecting groups. The coupling of phosphitylated protected peptides onto oligonucleotide-resins did not afford the desired nucleopeptide, which was nevertheless obtained after oligonucleotide elongation at the hydroxyl group of the resin-bound peptide and deprotection under mild basic conditions. A preliminary study on the stability of different nucleopeptides to bases is also reported.Keywords
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