IMMUNOENZYME HISTOCHEMICAL-LOCALIZATION OF FIBRIN DEGRADATION PRODUCTS IN TISSUES

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  • 1 January 1981
    • journal article
    • research article
    • Vol. 103  (3) , 337-344
Abstract
An immunoenzyme histochemical study was conducted to localize fibrin degradation products (FDP) in rat tissues during disseminated intravascular coagulation (DIC). Serial measurements of FDP levels in serum after thrombin-induced DIC showed peak levels at 30 min, the FDP were rapidly cleared from the circulation (half-life about 1 h). Rat tissues obtained from 10 min to 3 h after induction of DIC were studied immunohistochemically. A method was developed to differentiate FDP from fibrin in tissue sections. This method is based on the observation that in paraplast-embedded tissues, FDP can be demonstrated following ethanol fixation only, and that fibrin is demonstrable after both paraformaldehyde fixation and ethanol fixation. FDP will react to some of the antisera employed only, while fibrin will react to all antisera used (antisera against fibrin monomer, against the constituent chains of fibrinogen, and against FDP-D and -E). At 10-20 min after the induction of DIC, FDP were found in kidney proximal tubule epithelial cells. These FDP could be demonstrated using antiserum against the constituent chains of fibrinogen, but not by antisera against FDP-D or -E. At 30-90 min, FDP were found inside liver macrophages. The FDP in liver did not react to anti-chain antisera, though they did react to antisera against FDP-D and -E. Since no FDP were found in other tissues, rat FDP are apparently cleared by kidney (earlier phase) and liver (later phase) only. In human cases of DIC, FDP could be demonstrated in kidney proximal tubule cells and in liver macrophages as well.