Determination of Amitraz in Honey by First-Derivative Spectrophotometry

Abstract
A new method for the determination of 1,5-bis(2,4-dimethylphenyl)-3-methyl-1,3,5-triazopenta-1,4-diene (Amitraz) in honey by derivative spectrophotometry is proposed. Amitraz is extracted from aqueous honey solutions with CCI4 and it is determined between 0.1 and 1.5 μg. ml−1 from the first-derivative spectrum by measuring the peak amplitude at 332 nm (1D332), as the vertical distance from the peak to the baseline.