Nucleotide trapping at the ATPase site of myosin subfragment 1 by a new interthiol crosslinking.
- 1 April 1986
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 83 (7) , 2037-2041
- https://doi.org/10.1073/pnas.83.7.2037
Abstract
When myosin subfragment 1 derivatives in which the reactive sulfhydryl SH1 has been blocked react with N,N''-p-phenylenedimaleimide or 5,5''-dithiobis (2-nitrobenzoic acid), the reactive sulfhydryl group SH2 of the 20-kDa domain is crosslinked with a thiol of the 50-kDa domain of the heavy chain. The crosslink induces the stable trapping of a significant amount of Mg2+-nucleotide in the ATPase site.Keywords
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