DEMONSTRATION OF BURST-PROMOTING ACTIVITY OF RECOMBINANT HUMAN GM-CSF ON CIRCULATING ERYTHROID PROGENITORS USING AN ASSAY INVOLVING THE DELAYED ADDITION OF ERYTHROPOIETIN
- 1 January 1985
- journal article
- research article
- Vol. 66 (6) , 1479-1481
Abstract
We demonstrate through the use of an in vitro assay involving the delayed addition of erythropoietin that human recombination GM-CSF, cloned from a mature T cell line, Mo, clearly has burst-promoting activity (BPA) on peripheral blood erythroid progenitors at picomolar concentrations. Delay for up to 72 hours of the addition of erythropoietin to semi-solid methylcellulose cultures of concentrated peripheral blood progenitors minimizes or eliminates BPA-independent erythroid colony formation with little loss of BPA-dependent erythroid colony formation. This assay will prove useful in accurately detecting sources of BPA.This publication has 5 references indexed in Scilit:
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