Analysis of in vitro Translation of Bean Yellow Mosaic Virus RNA: Inhibition of Proteolytic Processing by Antiserum to the 49K Nuclear Inclusion Protein
- 1 May 1988
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 69 (5) , 1117-1122
- https://doi.org/10.1099/0022-1317-69-5-1117
Abstract
In vitro translation of bean yellow mosaic virus (BYMV) RNA in rabbit reticulocyte lysate without dithiothreitol (DTT) resulted in the accumulation of high Mr products. These were readily processed to low Mr mature proteins after the addition of DTT followed by a 2 h incubation. Immunoprecipitation analyses of the partially processed high Mr products with antisera to the 49K and 54K nuclear inclusion (NI) proteins, cylindrical inclusion (CI) protein, tobacco vein mottling virus helper component (HC) protein and capsid protein (CP) resolved the following BYMV RNA genome map: 5'' end, 32K unidentified protein, 48K HC, 42K unidentified protein, 73K CI, 49K NI, 54K NI, 32K CP, 3'' end. The addition of the 49K NI protein antiserum inhibited the proteolytic processing of the high Mr translation products. The inhibition of processing was diminished by diluting the 49K NI antiserum or by adding purified 49K NI protein. Preimmune serum or the antisera to the 54K NI, CI and capsid proteins were not as effective in inhibiting the proteolytic processing. These results provide evidence that the 49K NI protein or a related protein may have a protease function in the processing of the potyvirus polyproteins.This publication has 11 references indexed in Scilit:
- Potyviral proteins share amino acid sequence homology with picorna-, como-, and caulimoviral proteinsVirology, 1987
- The nucleotide sequence of the coding region of tobacco etch virus genomic RNA: Evidence for the synthesis of a single polyproteinVirology, 1986
- Cistron mapping of tobacco vein mottling virusVirology, 1986
- Sequence determination of the capsid protein gene and flanking regions of tobacco etch virus: Evidence for synthesis and processing of a polyprotein in potyvirus genome expressionProceedings of the National Academy of Sciences, 1985
- Translation of papaya ringspot virus RNA in vitro: Detection of a possible polyprotein that is processed for capsid protein, cylindrical-inclusion protein, and amorphous-inclusion proteinVirology, 1985
- Translation of soybean mosaic virus RNA in Vitro: Evidence of protein processingVirology, 1984
- Cell-free translation of tobacco vein mottling virus RNAVirology, 1983
- Strains of Bean Yellow Mosaic Virus Compared to Clover Yellow Vein Virus in Relation to Gladiolus Production in FloridaPhytopathology®, 1983
- Translation of potyvirus RNA in a rabbit reticulocyte lysate: Cell-free translation strategy and a genetic map of the potyviral genomeVirology, 1980
- Translation of potyvirus,rna in a rabbit reticulocyte lysate: Reaction conditions and identification of capsid protein as one of the products of in vitro translation of tobacco etch and pepper mottle viral RNAsVirology, 1980