Purified bovine AMH induces a characteristic freemartin effect in fetal rat prospective ovaries exposed to it in vitro
Open Access
- 1 May 1987
- journal article
- research article
- Published by The Company of Biologists in Development
- Vol. 100 (1) , 43-55
- https://doi.org/10.1242/dev.100.1.43
Abstract
To determine whether anti-Mullerian hormone (AMH) is responsible for the gonadal lesions observed in bovine genetic females united by placental anastomoses to male twins (freemartins), prospective ovaries of fetal rats were exposed to purified bovine AMH in vitro. In cultures initiated at 14 days p.c. and maintained 3 to 10 days, AMH consistently induced a characteristic ‘freemartin effect’, namely reduction of gonadal volume, germ cell depletion and differentiation, in the gonadal blastema, of epithelial cells with large clear cytoplasm linked by interdigitations, resembling rat fetal Sertoli cells. These cells tend to become polarized and form cords, delineated by a continuous basal membrane containing laminin and fibronectin. Such structures, resembling developing seminiferous cords, were not detected in control ovarian cultures. These data strongly suggest that AMH is the testicular factor responsible for triggering the morphological abnormalities of freemartin gonads.This publication has 32 references indexed in Scilit:
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