The regulation of amino sugar metabolism in Bacillus subtilis

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Abstract
Ultrasonic extracts of B. subtilis contain the enzyme L-glutamine-D-hexose 6-phosphate aminotransferase (L-glutamine-D-fructose 6-phosphate aminotrans-ferase, EC 2.6.1.16). It closely resembles the L-glutamine-D-fructose 6-phosphate aminotransferase of Escherichia coli. Ultrasonic extracts also contain low amounts of glucosamine-6-phosphate deaminase (2-amino-2-deoxy-D-glucose-6-phosphate ketol-isomerase, EC 5.3.1.10) which may be separated from L-glutamine-D-hexose-6-phosphate aminotransferase by precipitation with protamine sulphate. Growth of B. subtilis in the presence of amino sugars causes repression of L-glutamine-D-hexose 6-phosphate aminotransferase and concomitant induction of glucosamine-6-phosphate deaminase. The induction of glucosamine-6-phosphate deaminase is repressible by glucose.