pH‐independent inhibition of restriction endonuclease cleavage via triple helix formation by oligonucleotides containing 8‐oxo‐2′‐deoxyadenosine
- 21 November 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 355 (1) , 11-14
- https://doi.org/10.1016/0014-5793(94)01139-7
Abstract
The ability of homopyrimidine oligonucleotides containing 8-oxo-2′-deoxyadenosine to form stable, triple helical structures with the sequence containing the recognition site for the class II-S restriction enzyme, Ksp632-I, was studied as a function of pH. The 8-oxo-2′-deoxyadenosine-substituted oligomers were shown to inhibit enzymatic cleavage and to bind within the physiological pH range in a pH-independent fashion without compromising specificity.Keywords
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