Analytical and preparative electrophoresis in a nonuniform electric field
- 1 March 1988
- journal article
- research article
- Published by Wiley in AIChE Journal
- Vol. 34 (3) , 483-492
- https://doi.org/10.1002/aic.690340316
Abstract
A nonuniform electric field is proposed as the basis for the batch and continuous electrophoretic separation of biomolecules. A theoretical analysis shows the advantages of these techniques over conventional separation processes that use a uniform electric field. The mathematical modeling of such processes and its implications for the experimental design of a nonuniform field separator are presented.Keywords
This publication has 25 references indexed in Scilit:
- Electrophoretic Separations of Large DNA Molecules by Periodic Inversion of the Electric FieldScience, 1986
- Wedge‐Shaped ultrathin polyacrylamide and agarose gels for isoelectric focusing: A new method for typing phosphoglucomutase (PGM1) in semen stains and vaginal swabsElectrophoresis, 1985
- Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresisCell, 1984
- Mobility of DNA in gel electrophoresisBiopolymers, 1982
- Why does the electrophoretic mobility of DNA in gels vary with the length of the molecule?Biopolymers, 1982
- A method for continuous preparative electrophoresis using a supporting mediumElectrophoresis, 1981
- Continuous flow electrophoresis: The crescent phenomena revisited Part II: Nonisothermal effectsElectrophoresis, 1981
- Free-Flow Electrophoresis I. Theoretical and Experimental Investigations of the Influence of Mechanical and Electrokinetic Variables on the Efficiency of the MethodHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1975
- Design Theory and Separations in Preparative-Scale Continous-Flow Annular-Bed ElectropheresisIndustrial & Engineering Chemistry Process Design and Development, 1971
- XI.—Solution and pseudo-solution. Part IJournal of the Chemical Society, Transactions, 1892